Introduced: See Comments.
Number of Barks stories: 2*
FG1948 Donald in Toyland
CP1 Letter to Santa.

Comments:
The Christian bishop Saint Nicholas of Myra from the 4th century's Turkey is the primary inspiration, but today's character is traditionally supposed to have arisen from a Coca-Cola advertisement campaign in the 1930s. The company's character was built on an already existing figure drawn by Thomas Nast in 1863, January 3, though.

* Also seen in the last panel of CID 'The Black Pearls' as a narrator but not drawn by Barks.

Introduced: 1925, February 15 in Alice Solves the Puzzle (Disney live action/animated short).
Number of Barks stories: 4
FC0009 Pirate Gold
FC0079 The Riddle of the Red Hat
U$28 'The Inventors' Contest'
WDCS 'Attic Antics'.

Comments:
It may be argued that Barks used Pete in other stories as well, but in them he looked too different to be accepted here.

Introduced: 1928, November 18 in Steamboat Willie (Disney cartoon).
Number of Barks stories: 1
FC0079 The Riddle of the Red Hat.

Introduced: 1928, November 18 in Steamboat Willie (Disney cartoon).
Number of Barks stories: 1
FC0079 The Riddle of the Red Hat.

Introduced: 1929, June 28 in The Plow Boy (Disney cartoon).
Number of Barks stories: 1
FC1055 Donald's Party.

Introduced: 1930, April 1 in Death Valley (newspaper strip).
Number of Barks stories: 3
FC0079 The Riddle of the Red Hat
FC1055 Donald's Party
FC1150 A Sticky Situation.

Introduced: 1932, May 25 in Mickey's Review (Disney cartoon).
Number of Barks stories: 1
FC0079 The Riddle of the Red Hat.

Comments:
Goofy was not named in the first cartoons. Later he was called Dippy Dawg and Crazy Dog, until he settled on his current name.

Introduced: 1932, September 18 in a newspaper strip.
Number of Barks stories: 1
U$13 'Lightning Power'.

Comments:
Barks had to use Morty and Ferdy instead of the planned nephews due to special postal regulations at the time.

Introduced: 1933, May 27 in Three Little Pigs (Disney cartoon).
Number of Barks stories: 4
FC1010 The Flying Farm Hand
FC1073 Touché Toupée
FC1161 The Day the Farm Stood Still
FC1161 The Training Farm Fuss.

Comments:
His real name is Zeke Wolf.

Introduced: 1934, June 9 in The Wise Little Hen (Disney cartoon).
Number of Barks stories: Many(!), starting with FC0009 Pirate Gold.

Introduced: 1934, August 11 in Orphans Benefit (Disney cartoon).
Number of Barks stories: 2
FC1055 Double Date
FC1150 Undercover Girl.

Introduced: 1935, March 9 in I Haven't got a Hat (Warner cartoon).
Number of Barks stories: 1
FC0048 Porky of the Mounties.

Introduced: 1937, January 9 in Don Donald (Disney cartoon).
Number of Barks stories: 92, starting with WDCS036 The Mighty Trapper.

Introduced: 1937, April 3 in Porky's Romance (Warner cartoon).
Number of Barks stories: 1
FC0048 Porky of the Mounties.

Introduced: 1937, October 17 in a newspaper strip.
Number of Barks stories: Many(!), starting with FC0009 Pirate Gold.

Introduced: 1938, March 17 in a newspaper strip.
Number of Barks stories: 9, starting with WDCS043 Three Dirty Little Ducks.

Comments:
The initial name in the USA was Bolivar but the people of Colombia complained, since they had the freedom fighter Simon Bolivar as their hero. So the name was altered. Sometimes he was also called Borny and Bernie, though.

Introduced: 1939, May 09 in Donald's Cousin Gus (Disney cartoon).
Number of Barks stories: 17, starting with WDCS132 'Attic Antics'.

Comments:
Barks served as Story Director for the cartoon.

Introduced: 1939, June 10 in The Bear That Couldn't Sleep (MGM cartoon).
Number of Barks stories: 26, starting with OG11 'The Sound of His Horn'.

Comments:
For the first few years Barney was accompanied by a gopher until he teamed up with Benny Burro.

Introduced: 1939, September 9 in Life Begins for Andy Panda (Lantz cartoon).
Number of Barks stories: 1
NF76 'The Widow's Doughnuts'.

Introduced: 1940, July 27 in A Wild Hare (Warner cartoon).
Number of Barks stories: 1
FC0048 Porky of the Mounties.

Comments:
Barks could not get Bugs' facial expressions right, so all the faces were redrawn by his publisher.

Introduced: 1941, October 23 in Dumbo (Disney feature cartoon).
Number of Barks stories: 1
FC1010 The Flying Farm Hand.

Introduced: 1942, May 16 in Little Gravel Voice (MGM cartoon).
Number of Barks stories: 29 stories, starting with OG08 'With a Little Help to My Friends'.

Comments:
Benny is also referred to as The Lonesome Burro.

Introduced: 1943, March 20 in Dumb-hounded (MGM cartoon).
Number of Barks stories: 2
OG09 'A Hard Day's Day Off'
OG11 'Mother is a Wolf's Best Friend'.

Comments:
The character is known under several aliases such as Houdini Wolf, Killer Diller Wolf, Torpedo Wolfie Boy, and Lobo the Louse, but his prison number chiefly seemed to stick with him.

Introduced: 1943, March 20 in Dumb-hounded (MGM cartoon).
Number of Barks stories: 2
OG09 'A Hard Day's Day Off'
OG11 'Mother is a Wolf's Best Friend'.

Comments:
The character was later named Droopy.

Introduced: 1943, September 27 in a newspaper strip.
Number of Barks stories: 42, starting with FG1945 Donald's Best Christmas.

Comments:
It is quite obvious that Barks had no prior knowledge of Grandma as a character, when he first used her. In the story she is portrayed as a very youthful, shoeless girl wearing a bell-shaped hat.

Introduced: 1946, ? ? in a newspaper strip.
Number of Barks stories: 1
WDCS111 'Love Letters'.

Comments:
The character is normally referred to as the son of Bornworthy. In the story Donald affectionately calls him Behe.

Introduced: 1946, November 12 in Song of the South ( Disney live action/animated feature film).
Number of Barks stories: 1
FC1010 The Flying Farm Hand.

Introduced: 1950, February 15 in Cinderella (Disney animated feature film).
Number of Barks stories: 1
WDCS132 'Attick Antics'.

Comments:
The chubby mouse in the duo actually has another name that was given to him in the cartoon comic book from 1950. The young Cinderella addressed him: We'll name you Octavius ... but for short, we'll call you Gus! Barks only drew the duo in this story, where he called the mouse Gus-Gus, probably in order not to confuse him with Gus Goose who was also participating in the story.